The molecular epidemiological study on enteric adenovirus in stool specimens collected from wuhan area by using digoxigenin labeled DNA probes

Abstract

A summer-autumn (1994) molecular epidemiological study of enteric adenoviruses (EAds) in stool specimens collected in Wuhan area was conducted by using Digoxigenin-labelled DNA probes specific to EAd₄₀ and EAd₄₀ respectively. 44 of 602 specimens were positive, among which 23 cases were identified as EAd₄₀, 14 were EAd₄₀ infection and 7 were dual infection. The ratio of males to females for the positive specimens was 1. 44. The infection rate of EAd₄₀ and EAd₄₀ each displayed no marked difference in seasons (summer and autumn) and similar age distribution was found between them. All of the two types of EAds infections predominated in patients with diarrhea under 3 years old. The results indicated that the Digoxigenin probe could detect DNA quantities as low as 1 pg with satisfactory specificity and the technique can be used for both clinical and experimental purposes.

Keywords

digoxigenin-lablled probe / enteric adenoviruses / molecular epidemiology

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