Relationship between invasiveness of pituitary somatotrophinomas and structural abnormalities of protein kinase C gene in human

Abstract

The potential role of the protein kinase C (PKC) transduction system in controlling proliferation of human pituitary somatotrophinomas was investigated. Twenty somatotrophinomas were studied using PCR and diract sequencing methods. No point mutation within the αPKC gene, previously thought to be associated with invasive pituitary tumors, was found in any of the 20 somatotrophinomas. It is concluded that PKC transduction system may play an important role in controlling pituitary somatotrophinoma proliferation, but there is no correlation between invasiveness and the previously reported αPKC gene mutation.

Keywords

pituitary somatotrophinoma / protein kinase C / mutation

References

[1] AdmasE F, BrockmeierS, FriedmanE, et al.. Clinical and biochemical characteristics of acromegalic patients harboring gsp-positive and gsp-negative pituitary tumors. Neurosurgery, 1993, 33: 198-198

[2] AdamsE F, LeiT, BuchfelderM, et al.. Biochemical characteristics of human pituitary somatotropinomas with and without gsp mutations. In vitro culture studies. J Clin Endocrinol Metab, 1995, 80: 2077-2077

[3] AlvaroV, TouraineP, VozariR R, et al.. Protein kinase C activity and expression in normal and adenomatous human pituitaries. Int J Cancer, 1992, 50: 724-724

[4] AlvaroV, LevyL, DubrayC, et al.. Invasive human pituitary tumors express a point-mutated a-protein kinase C.. J Clin Endocrinol Metab, 1993, 77: 1125-1125

[5] BerridgeM J, IrvineR F. Inositol phosphates and cell signalling. Nature, 1989, 341: 197-197

[6] BurtonF H, HaselK W, BloomF E, et al.. Pituitary hyperplasia and gigantism in mice caused by a cholear toxia transgene. Nature, 1991, 350: 74-74

[7] FahlbuschR, BuchfelderM.. Current management of invasive pituitary adenomas. Contemp Neurosurg, 1989, 11: 1-1

[8] KargaH J, AlexanderJ M, Hedley-WhyteE T, et al.. Ras mutations in human pituitary tumours. J Clin Endocrinol Metab, 1992, 74: 914-914

[9] TodoT, BuchfelderM, ThieraufP, et al.. Immjnohistochemical expression of progein kinase C type I in human pituitary adenomas. Neurosurgery, 1993, 32: 635-635

[10] TodoT, FahlbuschR. Accumulation of inosital phosphates in low-passage human meningioma cells following treatment with epideminal growth factor. J Neurosurg, 1994, 80: 890-890