Immunohistochemical localization of fibronectin and laminin in murine liver using protein A-gold technique at light microscopic level — a methodological study

Abstract

An immurohistochemical staining combining protein A-gold(PAG) technique with silver intensification (PAGS) was employed to localize fibronectin (FN) and laminin (LN) in normal murine liver at light microscopic level. The technique possesses all the advantages from both Holgate’s IGSS method and PAG technique. It readily demonstrates FN and LN in formalin fixed, paraffin embedded tissue without enzymatic digestion. The procedure for staining FN and LN was optimized. Some technical points to note were discussed in detail.

Keywords

immunohistochemical staining / fibronectin / laminin / protein A-gold silver technique / murine liver

References

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