Effect of Anti-inflammatory Agent No.6 for injection on the cAMP content of rat peritoneal macrophages

Abstract

The effect of Anti-inflammatory Agent No.6 for injection (AI-6) on the cAMP content of rat peritoneal macrophages (Mϕ) was studied in the present work. It was demonstrated in in vitro studies that AI-6, while showing no effect on the basal cAMP level in resident peritoneal Mϕ, significantly facilitated the formyl Methionyl-Leucyl-Phenylalanine-induced increase in the intracellular cAMP concentration (control: 34.01 ±3.48 pmol/10⁷ cells, test; 41.68±2.76 pmol/10⁷ cells;P< 0.05). In vivo, AI-6 was found to increase the intracellular cAMP concentration of fetal calf serum(FCS)-elicited rat peritoneal Mϕ (control: 28.5±3.17 pmol/mg protein, test: 41.01±4.13 pmol/mg protein;P< 0.01). The possible effect of the drug-induced changes in cAMP level on Mϕ function is discussed.

Keywords

macrophage / traditional Chinese medicinal preparation / cAMP

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