An immunofluorescent assay for rapid detection of cytomegalovirus

Abstract

Two kinds of immunofluorescent assay, the indirect immunofluorescent assay (IIF) and the improved indirect immunofluorescent assay (improved-IIF), were used to detect the intracellular cytomegalovirus (CMV) antigens. It was found that the improved-IIF could demonstrate CMV antigens not only earlier but also in a smaller amount than the IIF. It is obvious that the improved-IIF is a more sensitive method than the IIF. The possible principles for the enhanced sensitivity of improved-IIF were discussed briefly.

Keywords

cytomegalovirus / indirect immunofluorescent assay / improved indirect immunofluorescent assay

References

[1] TheTH, et al.. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clin Exp Immunol, 1974, 16: 1-12

[2] GernaG, et al.. The immunoperoxidase technique for rapid human cytomegalovirus identification. Arch Virol, 1976, 50: 311-21

[3] 1983;97–127.

[4] GergelyL, et al.. Complement-fixing antibodies to human cytomegalovirus induced early nuclear antigens in mononucleosis. Med Microbiol Immunol, 1981, 170: 99-108