Changes of intracellular IP₃ with the expression of interleukin 2 receptor in human peripheral blood T lymphocytes

Abstract

The changes of the intracellular inositol-l,4,5-triphosphate (IP₃) associated with the expression of the interleukin 2 receptor (IL-2R) were studied. In resting lymphocytes, IL-2 did not alter intracellular concentration of IP₃, but Con A caused an increase in IP₃ by 45%. In IL-2 sensitive T cells, which expressed IL-2R by 83 %, the change of intracellular IP₃ was dependent upon IL-2 concentration. The IP₃ increased at IL-2 concentrations of 10 and 50 U/ml, and the maximal response of 60 % was found at the concentration of 50 U/ml. At IL-2 concentration of 100 U/ml no increase in IP₃ was observed. After binding of anti-Tac Me Ab to IL-2R of T lymphocytes the increase in IP₃ at IL-2 concentrations of 10 and 50 U/ml was significantly attenuated. It has been suggested that IL-2 could induce the changes of intracellular IP₃ of the human peripheral blood T cells, which is related to the IL-2 concentrations incubated with T cells and the expression of IL-2R on T lymphocyte.

Keywords

inositol-1,4,5-triphosphate / T lymphocyte / interleukin 2 / receptor

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