Human vascular endothelial growth factor cDNA cloning and expression in osteoblasts

Abstract

Human vascular endothelial growth factor (VEGF) cDNA was amplified by nested polymerase chain reaction method from the HL60 cells. Then a pCD-hVEGF₁₆₅ recombinant plasmid was constructed. Rabbit osteoblasts were transfected with pCD-hVEGF₁₆₅ plasmid by lipofectin mediated gene transfer. The transient expressive results were detected by immunohistochemical method. It was observed that the expression of human VEGF gene was detected 72 h after transfecting distinctly.

Keywords

vascular endothelial growth factor / gene amplification / gene expression

References

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