The clinic study on synchronous detection of HCV RNA in the plasma and PBMC of hepatitis C patients

Abstract

In order to increase the positive detection rate of HCV RNA in the patients with chronic hepatitis C (CHC), RT-PCR was used to synchronously detect HCV RNA in the plasma and peripheral blood mononuclear cells (PBMC) of 583 CHC patients with a continuously elevated level of ALT for more than one year. The results showed that the positive detection rate of HCV RNA in the plasma of the CHC patients was 19. 2 %, while 24. 5 % in PBMC. It was demonstrated that the positive detection rate for HCV RNA in PBMC was obviously higher than that detected in plasma. To synchronously detect HCV RNA in PBMC by using RT-PCR can increase the positive detection rate of HCV RNA in the CHC patients.

Keywords

hepatitis C / RT-PCR / plasma / peripheral blood mononuclear cells

References

[1] HellingsJ A, Van der Veen-du PrieJ, Snelting-Van DensenR, et al.. ZuckermannA J, et al.. Transmission of non-A, non-B hepatitis by leukocytes preparations. Viral Hepatitis and Liver Disease Viral, 1988New YorkAlan R Liss543-543

[2] TakamatsuK, RoyanagiY, OkitaR, et al.. Hepatitis C virus RNA in saliva. Lancet, 1990, 2: 1515-1515

[3] ZignegoA L, McchiaD, MontiM, et al.. Infection of peripheral mononuclear blood cells by hepatitis C virus. J Hepatol, 1992, 15: 382-382

[4] ChomocynskiG, SacchiN. Single-step method of RNA isolation by acid guanidium thiocyanate-phehol-chloroform extraction. Anal Biochem, 1987, 162: 156-156

[5] 1997, 24 (2):97

[6] ChangT T, YoungK C, YangY J, et al.. Hepatitis C virus RNA in peripheral blood mononuclear cell: comparing acute and chronic hepatitis C virus infection. Hepatlogy, 1996, 23: 977-977

[7] SanonnoD, IacobelliA R, CornacchiuloV, et al.. Detection of hepatitis C virus (HCV) proteins by immunofluorescence and HCV RNA genomic sequences by non-isotopic in situ hybridization in bone marrow and peripheral blood mononuclear cells of chronically HCV-infected patients. Clin Exp Immunol, 1996, 103: 414-414