Effect of Jianpiyiqi prescription on the expression of heat shock proteins in acetic acid-induced chronic gastric ulcer rats

Abstract

To investigate the effect of Jianpiyiqi prescription on the expression of heat shock proteins (HSPs) and the changes of HSPs in gastric-ulcerated rats, the rat model of chronic gastric ulcer was induced by acetic acid. The SABC immunohistochemical method was used to observe HSP70 of mucosa around the gastric ulcer. Imaging analysis was performed. Western dot blot was used to detect HSPs contents in the plasma and gastric mucosal homogenate in each group. The results showed that HSP70 contents of the mucosa around the gastric ulcer in the model group and ranitidine-treated group were increased as compared with control group (P< 0. 01). Jianpiyiqi could increase the expression of HSP70 of the mucosa around the gastric ulcer further as compared with that in the model group and ranitidine-treated group (P< 0. 01). The HSP70 contents in the serum and mucosa in the model group and ranitidine-treated group were increased as compared with control group (P< 0. 01,P< 0. 05 respectively). HSP70 of serum and mucosa in the Jianpiyiqi-treated group was higher than in the model group and ranitidine-treated group (P< 0. 05,P< 0. 01 respectively). It was concluded that HSP might play a role in the process of pathophysiology of gastric ulcer. Jianpiyiqi could enhance gastric ulcer-healing through the protective mechanism of HSPs.

Keywords

peptic ulcer / heat shock proteins / acetic acid / immunohistochemistry / western dot blot / Jianpiyiqi

References

[1] TakagiK, OkabeS, SazikiR. A new method for the production of chronic gastric ulcer in rats and several drugs on its healing. Jap J Pharmc, 1969, 19: 418-418

[2] Western 1996,14 (6): 376.

[3] HikakawiaT, RokutanK, NikawaT, et al.. Geranylgeranylacetone induces HSPs in cultured guinea pig gastric mucosa cells and rat gastric mucosa. Gastroenterology, 1996, 111: 345-345

[4] 1998,18(6):162

[5] YamaguchiH, OsakiT, TaguchiH, et al.. Induction and epitope analysis of Helicobacter pylori heat shock protein. J Gastroenterol, 1996, 31(9): 12-12

[6] KobayashiK, YokotaK, YoshinoT, et al.. Detection of Helicobacter pylori associated antigen and heat shock protein 60 on follicular dendritic cells in the germinal centres of low grade B cell lymphoma of gastric mucosa associated lymphoid tissue (MALT). J Clin Pathol, 1998, 51(5): 396-396

[7] BartonS G, WinrowV R, RamptonD S, et al.. Circulating antibodies to the 60-kD heat shock protein (hsp) family in patients with Helicobacter pylori infection. Clin Exp Immunol, 1998, 112(3): 490-490