ATP evokes calcium transients in single pulmonary artery endothelial cell in primary cultures

Abstract

Effects of ATP on cytosolic free calcium ([Ca²⁺]i) in single porcine pulmonary artery endothelial cell were studied. Using a dual-wavelength excitation microflurometry. it was found that ATP evoked a rapid transient in [Ca²⁺]i which was then followed by a maintained elevation of [Ca²⁺]i. The removal of extracellular Ca²⁺ abolished the maintained plateform, but exerted no obvious effect on the initial-transient. These results suggest that ATP stimulates both calcium release from intracellular calcium pool(s) and calcium influx across the plasma membrane from extracellular space.

Keywords

cytosolic free calcium / endothelial cell / ATP

References

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