Preliminary study on bcr rearrangement in leukemia

Abstract

Southern-blot analysis was conducted in 15 patients by using a 1. 2 Kb HindI /BglI fragment from the 3′ end of the bcr region and a 2. 0 Kb BglI /HindI fragment from the 5′ end of the bcr as probes. Of the 15 patients in our group, 11 with chronic, myelocytic leukemia (CML), 3 with Ph-negative acute lymphocytic leukemia (ALL), one with myelodysplastic syndrome (MDS). Bcr rearrangements were detected in 9 patients with CML and were negative in the rest of the patients. The results showed that the identification of bcr rearrangement was very important in the diagnosis of Ph-positive leukemias.

Keywords

Southern blot analysis / bcr rearrangement / leukemia

References

[1] RowleyJ D. A new consistent chromosomal abnormality in chronic myelogenous leukemia identified by quinacrine fluorescence and Giemsa staining. Nature, 1973, 243: 290-290

[2] GroffenJ, StephensonJ R, HeisterkampN, et al.. Philadelphia chromosomal breakpoints are clustered within a limited region bcr on chromosome 22. Cell, 1984, 36: 93-93

[3] ShtivelmanE, LifshitzB, GaleR P, et al.. Fused transcript of abl and bcr genes in chronic myelogenous leukemia. Nature, 1985, 315: 550-550

[4] ChenS J, ChenZ, GrauszJD, et al.. Molecular cloning of a 5′-segment of the gemomic ph1 gene defines a new breakpoint cluster region (bcr 2) in Philadelphia-positive acute leukemias. Leukemia, 1988, 2(10): 634-634

[5] ManiatisT. Molecular Cloning, 1982U. S. ACold Spring Harbor

[6] FeyM F, PilkingtonS P, SummersC, et al.. Molecular diagnosis of hematologic disorders using DNA from stored bone marrow slides. Br J Haematol, 1987, 67: 489-489

[7] LornaM W, GarsonO M. Fluorodeoxy-uridine synchronization of bone marrow culture. Cancer Genet Cytogenet, 1983, 8: 123-123

[8] BlennerhassettG T, FurthM E, AndersonA, et al.. Clinic evaluation of a DNA probe assay for the Philadelphia (Ph1) translocation in chronic myelogenous leukemia. Leukemia, 1988, 2(10): 648-648

[9] ShtalridM, TalpazM, KurzrockR, et al.. Analysis of breakpoints within the bcr gene and their correlation with the clinical course of Philadelphia-positive chronic myelogenous leukemia. Blood, 1988, 72(2): 485-485