Establishment and application of SPA-co-operated ELISA for detection of anti-HCV-IgM

Abstract

A staphylococus aureus protein A co-operated ELISA (SPA-ELISA) for the detection of anti-HCV-IgM has been established using HCV antigenic polypeptide, SPA-bearing germs and horseradish peroxidase labelled anti-human IgM. The specificity of SPA-ELISA has been confirmed by some substitution tests, blocking tests and destroying test with 2-mercaptoethanol. The results showed that the rate of anti-HCV-IgG in a group of patients with acute hepatitis and there were significant difference in anti-HCV-IgM was higher than that of anti-HCV-IgM detected rates between patients with acute hepatits and those with chronic hepatitis (32.26%,P<0.01). On the other hand, the positive rates of anti-HCV-IgM were 53.66 % and 63.41 % in transfusion associated hepatitis, 38.10 % and 42.86 % in sporadic hepatitis, 6.11 % and 16.33 % in people who have had active social activities, 40.00 % and 10.00% in a group of blood donors respectively. Furthermore, taking into account the characteristics of HCV polypeptide used, its easiness of manipulation, and elimination of the interference of anti-HCV-IgG in sera, the new SPA-ELISA is believed to be of practical value in clinical and epidemiological studies of hepatitis C.

Keywords

hepatitis C / anti-HCV-IgM / SPA / ELISA

References

[1] KuoG, et al.. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science, 1989, 244: 362-362

[2] OkamotoH, et al.. Antibodies against synthetic oligopeptides deduced from the putative core for the, diagnosis of hepatitis C virus infection. Hepatology, 1992, 15: 180-180

[3] JeffersI J, et al.. Prevalence of antibodies to hepatitis C among patients with cryptogenic chronic hepatitis and cirrhosis. Hepatology, 1992, 15: 187-187

[4] HarveyJ, et al.. New kits on the block: Evaluation of second generation assays for detection of antibody to the hepatitis C virus. Hepatology, 1992, 15: 350-350

[5] JuanA Q, et al.. IgM antibody to hepatitis C virus in acute and chronic hepatitis C. Hepatology, 1991, 14: 38-38

[6] BrilliantiS, et al.. Significance of IgM antibody to hepatitis C virus in patients with chronic hepatitis C. Hepatology, 1992, 15: 998-998

[7] SjögrenM, et al.. Immunoglobulin M antibody to hepatitis B core antigen in patients with chronic type B hepatitis. Gastroenterology, 1985, 89: 252-252