Nitric oxide synthase activity in arterial tissues of cirrhotic rats

Abstract

The changes in the activities of constitutive nitric oxide (NO) synthase (cNOS) and inducible NOS (iNOS) were investigated in arterial tissues of CC1₄-induced cirrhotic adult SD rats. The aortic tissue homogenate were prepared in normal and cirrhotic rats. NOS activity was measured by conversion of³H-arginine to³H-citrulline. The activities of cNOS and iNOS were calculated in terms of presence or absence of Ca²⁺. The results showed that activities of total NOS, cNOS and iNOS in arterial tissues were all increased significantly in cirrhotic rats as compared with those in normal controls. There was a significant positive correlation between the activities of total NOS and cGMP content in cirrhotic arterial tissues.

Keywords

nitric oxide synthase / arterial tissue / cirrhosis / rats

References

[1] MengM C, LinT C, TsaiY T, et al.. Relationships between the severity of cirrhosis and haemodynamic values in patients with cirrhosis. J Gastroenterology and Hepatology, 1994, 9: 148-148

[2] GroszmannR J. Hyperdynamic circlation of liver diseases 40 years later: pathophysiology and clinical consequences. Hepatology, 1994, 20: 1359-1359

[3] VallanceP, MoncadaS. Hyperdynamic circulation in cirrhosis: a role for nitric oxide. Lancet, 1991, 337: 776-776

[4] EvansT, CarpenterA, CohenJ. Purification of a distinctive form of endotoxin — induced nitric oxide synthase from rat liver. Proc Natl Acad Sci USA, 1992, 89: 5361-5361

[5] StarkM E, SqurzewskiJ H. Role of nitric oxide in gastrointestinal and hepatic function and disese. Gastroenterology, 1992, 103: 1928-1928

[6] BugaG M, GoldM E, FukutoJ M, et al.. shear stress-induced release of nitric oxide from endothelial cells grown on beads. Hepertension, 1991, 17: 187-187

[7] KelmM, FeelischM, DeussenA, et al.. Release of endothelium derived nitric oxide in relation to pressure and flow. Cardiovascular Research, 1991, 25: 831-831

[8] CahillP A, WuY P, SitzmannJ V. Nitric oxide synthase activity in portal hypertension. Hepatology, 1993, 18: 141A-141A

[9] MehtaR, GottsteinJ, ZelleerW P, et al.. Endotoxin and the hyperdynamic circulation of portal vein-ligated rats. Hepatology, 1990, 12: 1152-1152