Quantitative analysis of glomeruli lesions in patients with mesangial proliferative glomerulonephritis

Abstract

By using computer imaging analysis system combined with light microscopy, the glomeruli lesions on biopsy specimens sections were quantitatively analysed. The patholoical changes of mesangial proliferative glomerulonephritis (MsPGN) in children were quantitatively evaluated and the correlation between the mesangial matrix area (MA) and some clinical data were also analysed. The results indicated that the levels of various glomerular parameters in MsPGN group were higher than those of normal controls. No correlation was found between MA and 24 h urinary protein excretion, but a negative correlation was revealed between MA and estimated GFR. MA was also correlated with the duration of MsPGN recovery. It was suggested that the quantitative analysis of glomerular parameters by computer is a reproducible methods. The parameter of MA may be used for evaluation of the renal function, determination of the duration of therapy and evaluation of prognosis of MsPGN in children.

Keywords

quantitative analysis / computer imaging analysis system / glomerular parameters

References

[1] WyattR J, JulianB A. Iga nephnopathy: Presentation clinical course and prognosis in children and adults. Am J Kindney Dis, 1984, 4: 192-192

[2] LevyM. Berger’s disease in children: natural history and outcome. Medicine, 1985, 64: 157-157

[3] SuZukiJ, YoshikawaN, NakamuraH. A quantitative analysis of the mesangium in children with IgA nephropathy: Sequential study. J Patholo, 1990, 161(1): 57-57

[4] SchwartzG J. The use of plasma creatinine concentration for estimating glomerular filtration rate in infants, children and adolescents. Pediatr Clin North Am, 1987, 34: 571-571

[5] SuzukiJ, YoshikawaN, NakamuraH. A quantitative analysis of glomeruli in focal segmental glomerulosclerosis. Pediatr Nephrol, 1994, 8(4): 416-416

[6] NybergE. Glomerular volume and renal function in children with different type of nephrotic syndrome. Pediatr Nephrol, 1994, 8: 285-285

[7] TatenoS, KobayashiY. Quantitative analysis of mesangial areas in serial biopsied patients with IgA nephropathy. Nephron, 1987, 46: 28-28

[8] D’AmicoG. Idiopathic IgA mesangial nephropathy correlation of clinical and histologic features. Lab Invest, 1983, 48: 19-19

[9] Croker. IgA nephropathy clinical and histological study of 374 patients. Medicine, 1985, 64: 49-49

[10] DrozD, KramarA, NawarT, et al.. Primary IgA nephropahty: prognostic factor. Contrib Nephrol, 1984, 40: 202-202

[11] D’AmicoG, MinettiL, PonticelliC, et al.. Prognostic indicators in idiopathic IgA mesangial nephropathy. Q J Med, 1986, 59: 363-363

[12] WaldoF B. Treatment of IgA nephropathy in children: efficacy of alternate-day oral prednisone. Pediatr Nephrol, 1993, 7: 529-529