Effect of captopril on antithrombus function of endothelium

Abstract

The experimental models of cultured porcine aortic endothelial cells (EC)in vitro were established. 6-keto-prostaglandin F_1a (6-keto-PGF_1a), thromboxane B₂(TXB₂)content, plasminogen activator (PA), plasminogen activator inhibitor (PAD activity in cultured medium and cyclic adenosine monophosphate (cAMP) level in EC were measured with radioimmunoassay (RIA), chromogenic substrates methods, in order to assess the effect and mechanism of captopril (CP) on antithrombus function of EC. The results showed that after administration of CP, the contents of 6-keto-PGF_1a and cAMP and PA activity were significantly higher, PAI activity were remarkably lower than those of control group. These effects were dose-dependent. Our finding indicated that CP might act as a prospective drug for antithrombosis through promoting anticoagulation and fibrinolysis function and increasing antithrombus action of EC.

Keywords

captopril / endothelium / cultured cell

References

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