Detection of surface antigen expression of pre- and postcultured normal adult’s bone marrow cells by using ABC technique and its significance

Abstract

The expression of surface antigens on both normal adult’s bone marrow and multipotential progenitor cells (CFU-Mix) was detected by use of ABC technique. The culture system of CFU-Mix was an ideal model for studying the differentiation direction of hematopoietic cells. The monoclonal antibodies (McAb) CD₃, CD₄, CD₈specific for T-lymphocytic lineage, CD₂₂ for B-lymphocytic lineage and CD₁₁, CD₁₃, CD₁₄ for granulocytomonocytic lineage were all demonstrated in certain proportion in cultured CFU-Mix, which might be associated with the control of hematopoiesis. A decrease in the positive rate of OKT, and CD₃₄ in cultured cells might be related to the differentiation of hematopoietic cells. SZ-2 and SZ-21 could contribute to the identification of the presence of megakaryocytes in CFU-Mix.

Keywords

ABC technique / bone marrow cell / CFU-Mix / monoclonal antibody

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