Effects of Isophenphos-I on the respiratory function of rat liver mitochondria

Abstract

The effects of insecticide Isophenphos-I on respiration of rat liver mitochondria were studied. It was found that Isophenphos-I inhibited the respiration of mitochondria both in vivo and in vitro, and reduced the activities of glutamate dehydrogenase, succinate dehydrogenase, succinatecytochrome C reductase and Mg⁺⁺-ATPase in vitro. The effects of the insecticide on the permeability and lipid peroxidation of the mitochondrial membrane were also investigated in vitro in order to explore the possible mechanism of the respiratory inhibition of mitochondria by the insecticide.

Keywords

liver mitochondria / insecticide / cytochrome oxidase / succinate dehydrogenase / glutamate dehydrogenase / succinate-cytochrome C reductase / NADH dehydrogenase / Mg⁺⁺-ATPase

References

[1] SkoniecznaM, et al.. Modification of oxidative phosphorylation in brain mitochondria during development and aging by the oxygen analog of ronnel. Pestic Biochem Physiol, 1980, 14: 314-8

[2] HeidkerJC, PardiniRS. Inhibition of mitochondrial electron transport by guthion, some related insecticides and degradative products. Bull Environ Contam Toxicol, 1972, 8: 141-6

[3] SitkiewiczD, et al.. The effect of ronnel and its oxygen analog on succinate oxidation by rat brain, liver homogenates and mitochondria. Pestic Biochem Physiol, 1977, 7: 501-9

[4] Gazzoti P, et al. Preparation and assay of animal mitochondria and submitochondrial vesicles. In Carafoli, E and Demenza, G. eds. Membr Bioch emBerlin: Springer, 1979: 62–76.

[5] LapercheY, OudeaP. Inhibition by sulfobromophthalein of mitochondrial translocation of anions and adenine nucleotides. J Pharmacol Exp Ther, 1976, 197: 235-44

[6] NishiharaY. Effects of polychlorinated biphenyls on isolated rat liver mitochondria. Arch Environ Contam Toxicol, 1983, 12: 517-22

[7] SottocasaGL, et al.. An electron-transport system associated with the outer membrane of liver mitochondria. J Cell Biol, 1967, 32: 415-38

[8] OhyamaT, et al.. Effects of dichlorodiphenyltrichloroethene and its analogues on rat liver mitochondria. Biochem Pharmacol, 1982, 31: 397-404

[9] PedersenPL, et al.. Preparation and characterization of mitochondria and submitochondrial particles of rat liver and liver-derived tissues. Methods Cell Biol, 1978, 20: 411-81

[10] SoileauSD, MorelandDE. Effects of chlordecone and its alteration products on isolated rat liver mitochondria. Toxicol Appl Pharmacol, 1983, 67: 89-99

[11] ManringJA, MorelandDE. Effects of chlordecone on isolated rat liver mitochondria: Effects upon liver adenosine triphosphate and possible correlation with inhibition of bile flow in the rat. Toxicol Appl Pharmacol, 1981, 59: 483-8

[12] OhkawaH, et al.. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 1979, 95: 351-8

[13] LowryCH, et al.. Protein measurement with the folin phenol reagent. J Biol Chem, 1951, 193: 265-75