Immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding section of the bone marrow from patients with myelodysplastic syndrome and its clinical implication

Abstract

An immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding bone marrow sections obtained from 10 patients with myelodysplastic syndrome (MDS) was presented. The results of qualitative and quantitative immunohistochemical analysis are as follows: (1) Labile antigens of T lymphocytes were well preserved, thus allowing analysis of distribution of T lymphocyte subsetsin situ; (2) the average number of T³, T⁴ and T⁸ lymphocyte of the diffuse infiltrate was about 2%, 0.4%, 0.5%, respectively, of all nucleated cells in bone marrow, and T⁴/T⁸ of T cells were below 1.0 in patients with MDS; (3) there were cases of RAS showing T lymphocyte aggregation in bone marrow, but no patient exhibited progressive refractory anemia with excess of blasts(RAEB) and RAEB in transformation (RAEBT). These findings indicated that the immunological abnormalities are of importance in the evaluation of pathogenesis and prognosis of MDS.

Keywords

immunohistochemistry / plastic embedding of bone marrow / distribution of T cell subsetsin situ / myelodysplastic syndrome

References

[1] BennettJ M. Classification of the myelodysplastic syndromes. Clin Haematol, 1986, 15: 909-909

[2] 1993, 22:77

[3] CordellJ L, FaliniB, ErberW N, et al.. Immunoenzymatic labelling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (A-PAAP complexes). J Histochem Cytochem, 1984, 32: 219-219

[4] HarveyD M R. Freeze-substitution. J Microsc, 1982, 127: 209-209

[5] ClarkP, NormanseilD E, InnesD J, et al.. Lymphocyte subsets in normal bone marrow. Blood, 1986, 67: 1600-1600

[6] ColombatP H, RenouxM, LamagnereJ P, et al.. Immunologic indices in myelodysplastic syndromes. Cancer, 1988, 61: 1075-1075

[7] KristenseJ S, HoklandP. Monoclonal antibody ratios in malignant myeloid diseasese: diagnostic and prognostic use in myelodysplastic syndromes. Br J Hematol, 1990, 74: 270-270

[8] MuftiG J, StevensJ R, OscierD G, et al.. Myelodysplastic syndromes: a scoring system with prognostic significance. Br J haematol, 1985, 59: 425-425