A sensitive HPLC technique for the quantitation of dauricine

Abstract

To establish the determination method of dauricine (Dau) concentration in rats’ blood and other biological samples, a reverse-phase HPLC method was adopted. Under the given condition, dauricine could be well separated. The retention time (t_R) of Dau and its internal standard, daurisoline were 9.2 and 6.1 respectively. The detection limit was 10⁻² mg/ml. The absolute recoveries of all kinds of samples were above 70 %, and the relative ones were over 85 %. A good liner relationship has been obtained over the entire range of 0.030 to 3.000 mg/L in blood samples and 0.050 to 5.000 mg/L in other tissue samples. The intraday and interday coefficients of variation were below 10 %. The results showed that the method can be used for detecting Dau in all kinds of biological samples.

Keywords

dauricine / RP-HPLC / concentration determination

References

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