Development of a surface acoustic wave LB membrane immunity sensor

Abstract

A new kind of biosensor for immunology was developed by ultrasonic technique and LB membrane. A double delay-line resonator was made by using ST-cut quartz crystal with working frequency of 149. 7 MHz. Then a layer of LB membrane was covered on it. When anti-IgM antibody of various concentrations was added to it, the sensor can be used to detect IgM antigen. The biosensor was highly sensitive, small and light. The experimental results showed that the working frequency change of the sensor was proportional to the concentration of antibody with its dilution ratio between 1: 10000 and 1: 100. It was also first observed that the frequency curve of the sensor resulting from the reaction of IgM antigen and antibody undulated in the experiment.

Keywords

sensor / immunology / surface acoustic wave / LB membrane

References

[1] Vo-DinhT, HouckK, StokesD L. Surface-enhanced Raman gene probes. Anal Chem, 1994, 66: 3379-3379

[2] ChangS M, TamiyaE, KarubeI. Chemical vapour sensor using a SAW resonator. Biosensors and Bioelectronics, 1991, 6: 9-9

[3] RoedererJ E, BastiadnsG J. Microgravimetric immunoassay with piezoelectric crystals. Anal Chem, 1983, 55: 2333-2333

[4] YoshioO, HiroshiE. Absorption behaviors of surfactant molecules on a lipid-coated quartz-crystal microbalance. Anal Chem, 1991, 63: 203-203

[5] WolfgngM H, MarassiF M, KalluryK M R, et al.. Surface acoustic wave sensor response and molecular modeling: selective binding of nitrobenzene derivatives to (aminopropyl) triethoxysilane. Anal Chem, 1990, 62: 32-32