Screening of novel epilepsy-related genes and isolation and identification of cDNAs

Abstract

Twenty cDNA differential fragments were isolated from the hippocampus of rats in epileptic state using mRNA differential display technique. Four fragments were sequenced and compared with the known sequences in the Genebank, which showed that ERG8, ERG11, ERG12 had no significant identity to any known sequences; ERG14 had 64 %– 69 % identity to microtubulin-associated protein of the rat. Because the differential expression of these genes was caused by epilepsy inducer coriaria lactone (CL) and anti-epilepsy drug MK-801 and ERG8 might be a novel candidate epilepsy gene; ERG11 and ERG12 might be novel candidate anti-epilepsy genes. Since the microtubulin-associated protein is closely associated with the collateral sprouting of mossy fibers in the hippocampus of seizured rat, the high expression of ERG14 in the early stage of epilepsy might predict the growth of axon and formation of synapse.

Keywords

mRNA differential display / epilepsy-related gene / molecular cloning

References

[1] GABA_A 1995, 26(4):351

[2] WangX, FeuersteinG Z. Direct sequencing of DNA isolated from mRNA differential display. Biotechniques, 1995, 3: 448-448

[3] LiangP, ZhuW, ZhangX, et al.. Differential display using one-base anchored oligo-dT primers. Neuleic Acids Res, 1994, 22: 5763-5763

[4] HikiK, D’AndreaR J, FurzeJ, et al.. Cloning, characterization and chromosomal location of a novel human K⁺-C1^- cotransporter. J Biol Chem, 1999, 274: 10661-10661

[5] BeckerA J, GillardonF, BlumckeI, et al.. Differential regulation of apoptosis-related genes in resistant and vulnerable subfields of the epileptic hippocampus. Brain Res Mol Brain Res, 1999, 67: 172-172

[6] BabityJ M, ArmstrongJ N, PlumierJ C, et al.. A novel seizure-induced synaptotagmin gene identified by differential display. Proc Natl Acad Sci USA, 1997, 94: 2638-2638

[7] RepresaA, BenA Y. Molecular and cellular cascades in seizure-induced neosynapse formation. Adv Neurol, 1997, 12: 25-25