Bcl-1 rearrangement and cyclin D1 protein expression in multiple myeloma precursor cells

Abstract

The rearrangement of Bcl-1 gene (Bcl-1/IgH rearrangement) and expression of cyclin D1 in multiple myeloma (MM) precursor cells were studied and the role of cyclin D1 in the pathogenesis of MM was investigated. The BCL-1 rearrangement and cyclin Dl protein expression in 15 cases of MM were detected. By using hemi-nested polymerase chain reaction (PCR) the genomic DNA from fresh peripheral blood and bone marrow was amplified and the expression of cyclin D1 in the smears was detected by using immunohistochemical method. Ten volunteer with normal bone marrow served as control group. The results showed Bcl-1 rearrangement was detectable in 3/15 (20%) MM pa- and cyclin D, expression in 4/15 (27 %) MM patients. Bcl-1 rearrangement and cyclin Dl protein expression were also detected in MM precursor cells. No overexpression of cyclin Dl or the rearrangement of the Bcl-1 gene was found in the 10 volunteers. It was concluded that Bcl-1 rearrangement and cyclin Dl protein overexpression were detected in MM precursor cells, speculating that overexpression of cyclin Dl protein may play an initial (critical) role in the pathogenesis of MM.

Keywords

multiple myeloma / BCL-1/IgH rearrangement / Clone cell / cyclin D1 protein

References

[1] BergsagelP L, NardiniE, BrentsL, et al.. IgH translocations in multiple myeloma: a nearly universal event that rarely involves c myc. Curr Top Microbiol Immunol, 1997, 224: 283-283

[2] VasefM A, MedeirosL J, YospurL S, et al.. Cyclin D1 protein in multiple myeloma and plasmacytoma: an immunohistochemical study using fixed, paraffin-embedded tissue sections. Mod Pathol, 1997, 10: 927-927

[3] RimokhR, BergerF, DelsolG, et al.. Detection of the chromosomal translocation t(11,14) by polymerase chain reaction in mantle cell lymphoma. Blood, 1994, 83: 1871-1871

[4] TaishitaM, KosakaM. Multiple myeloma: new evidence and insights from the immunoglobulin heavy chain gene and phenotypes. Leuk Lymphoma, 1995, 19: 395-395

[5] BakkusM H, Van RietI, De GreefC, et al.. The clonogenic precurser cell in multiple myeloma. Leuk Lymphoma, 1995, 18(3): 221-221

[6] TaishitaM, KosakaM, GotoT, et al.. Cellar origin and extent of clonal involvement in multiple myeloma: genetic and phenotypic studies. Br J Haematal, 1994, 87: 735-735

[7] BartkovaJ, LukasJ, StraussM, et al.. Cell-related variation and tissue restricted expression of human cyclin D1 protein. J Pathol, 1994, 172: 237-237