Preliminary study of the in vitro growth inhibition of human bladder cancer cell line BIU-87 by arsenic trioxide

Abstract

To study the effects of arsenic trioxide (As₂O₃) on the in vitro growth of human bladder cancer cells and the mechanisms. The growth inhibition rates of human bladder cancer cell line BIU87 by various concentrations of As₂O₃ were detected by using MTT method. Cell apoptosis was detected by in situ terminally labeled transferase technique and bcl-2 gene expression of BIU-87 cells was observed by SABC immunohistochemical method. The results showed that As₂O₃ could inhibit the growth of BIU-87 effectively in a dose-dependent manner. After drug’s action, the apoptotic bladder cancer cells were obviously increased, which depended on the prolongation of the action time and Bcl-2 expression of BIU-87 cells was decreased significantly. It was suggested that As₂O₃ could significantly inhibit the growth of bladder human cancer cells. Inducing cell apoptosis by down-regulating the expression of bcl-2 gene might be one of its action mechanisms.

Keywords

arsenic trioxide / bladder cancer cell / apoptosis

References

[1] 1992,12:1702

[2] 1997,18:250

[3] MengZ Q.. Effects of arsenic on DNA synthesis in human lymphocytes. Arch Environ Contain Toxicol, 1993, 25: 525-525

[4] ChenG Q, ZhuJ, ShiX G, et al.. As₂O₃ induces NB4 cell apoptosis with down-regulation of bcl-2 gene and modulation of PML-RARa proteins. Blood, 1996, 88: 1052-1052

[5] CortezD, StociaG, PierceJ H, et al.. The BCR-ABL tyrosine kinase inhibits apoptosis by activating a Ras-dependent signaling pathway. Oncogene, 1996, 13: 2589-2589