Cd4⁺ T cell apoptosis induced by anti-CD4 antibodies

Abstract

To explore the inhibitory effects of anti-CD4 human/murine chimeric antibodies on lymphocyte proliferation, CD4⁺ T cell apoptosis induced by anti-CD4 antibodies was examined. Annexin-V-FITC and PI double stain method was employed to qualitatively and quantitatively determined CD4⁺ T cell apoptosis induced by anti-CD4 antibodies. Our results showed that anti-CD4 chimeric antibodies could specifically induce CD4⁺ T cell apoptosis. The ability of anti-CD4 chimeric antibodies to induce CD4⁺ T cell apoptosis was related with the presence of monocytes. It is concluded that the further cross-linking of anti-CD4 antibodies is important for inducing CD4⁺ T cell apoptosis.

Keywords

human/murine chimeric antibodies / CD4⁺ T cells / apoptosis

References

[1] KatsikisP D, WunderlichE S, SmithLCA, et al.. Fas Ag Stimulation Induces Maked Apoptosis of T Lymphocytes in Human Immunodeficiency Virus-Infected Individuals. J Exp Med, 1995, 181: 2029-2029

[2] SussG, ShortmanK A. Subclass of Dendritic Cells Kills CD4⁺ T Cells via Fas/Fas-ligand-induced Apoptosis. J Exp Med, 1996, 183: 1789-1789

[3] 1998.288–295

[4] VermesI, HaanenC, Steffens-NakkenH, et al.. A novel assay for apoptosis flow cytometric detection of phosphatidylerine expression on early apoptotic cells using fluorescein labelled Annexin V.. J Immunol Methods, 1995, 184(1): 395-395

[5] 1998.275–282

[6] WenR, SurmanS, BlackmanM A, et al.. The Conventional CD4⁺ T cell Response to Staphylococcal Enterotoxin B is Modified by its Superantigenic Activity. Cell Immunol, 1997, 176(2): 166-166