Study on the correlation of plasma NO, ET-1 and ALT in the patients with chronic hepatitis and cirrhosis

Abstract

The levels of plasma nitric oxide (NO), endothelin-1 (ET-1) and ALT in the patients with chronic hepatitis B and active cirrhosis and the correlation among them were observed and analyzed. NO₃⁻ was restored by using cadmium column assay and NO₂⁻ measured by heavy nitrogen assay. The primitive NO₃⁻ and total restored NO₂⁻ (NO₃⁻ / NO₂⁻) in plasma of the patients with chronic hepatitis and cirrhosis. Plasma ET-1 and ALT levels were determined by using radioimmunological assay and Lai’s assay, respectively. Compared with normal control group, the plasma levels of NO₂⁻ /NO₃⁻ and ET-1 in the patients with chronic active hepatitis and active cirrhosis were significantly increased (P < 0.05 – 0.01). There was a positive correlation between NO and ALT, and ET-1 and ALT in the patients with chronic active hepatitis and active cirrhosis respectively. It was suggested that elevation of both NO and ET-1 levels were closely related with injury severity of liver function.

Keywords

chronic hepatitis B / active hepatic cirrhosis / nitric oxide / endothelin / ALT

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