Inhibitory Effect of recombinant FN polypeptide CH50 on the metastasis and tumor growth

Abstract

The inhibition of CH50, a recombinant polypeptide of human fibronectin, on the formation of tumor metastasesin vivo was studied by inoculation of melanoma B16/F1 cells by hypodermic or intraperitoneal injection, and the size and amount of tumor nodes after therapy were measured. In the treatment of hypodermic tumor, local injection of CH50 produced much better efficacy than distance injection of CH50 did. The inhibitory effect of CH50 on the growth of tumor reached 50 %. In the treatment of peritoneal metastasis, the inhibition of CH50 on metastases smaller than 1 mm was above 80 %, and above 50 % on metastases larger than 1 mm. A better efficacy was achieved if CH50 was used in combination with hydroxycamptothecine (HCPT), a chemotherapeutic agent. CH50 displayed a strong inhibitory effect on the formation of small metastasis and growth of larger nodes of tumor, suggesting that CH50 plays a very important role in combined treatment of tumor therapy.

Keywords

fibronectin / recombinant polypeptide / tumor / metastasis

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