Diagnosis of disorders of platelet function by flow cytometry

Abstract

The platelet membrane glycoprotein (GP) II_b/III_a, GPIX were detected by immunogold assay and flow cytometry, respectively, in two cases of Glannzmann’s thrombasthenia (GT). The immunogold assay showed that the GP II_b/III_a. gold granules in GT were decreased obviously, compared with that in normal controls. Flow cytometry results showed that GP II_b/ III_a in two cases of GT was 0. 1 % and 0. 5% of normal control, respectively. While GPIX showed no difference between the GT and normal control. The patients’ father showed no bleeding symptoms, whose GP II_b/III_a accounted for 36. 99% of normal control. The experiment results suggested that flow cytometry is a quick, simple and sensitive method for the diagnosis of GP disorders.

Keywords

flow cytometry / platelet membrane glycoprotein / Glannzmann’s thrombasthenia

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