Effect of diallyl trisulfide on the activation of T cell and macrophage-mediated cytotoxicity

Abstract

At high concentration (50 μg/ml), diallyl trisulfide (DATS) had an inhibitory effect on T cell activation (compared with control group,P<0.05). But at appropriate concentrations (3.125–12. 5 μg/ml), DATS augmented the activation of T lymphocytes by Con A (compared with control group,P<0. 01). The augmentation of T cell activation by DATS was related to its inhibitory effect on the production of nitric oxide (NO) by macrophages. In a wide range of concentrations (1–100 μg/ml), DATS can inhibit the production of NO by macrophages (P<0.05,P<0.01). In addition, DATS can antagonize the inhibition of tumor-derived immunosuppressive factors produced by S180 cells and Ehrlich ascitic cancer cells on the activation of T cells, and reduce the inhibitory rate significantly (P < 0.01). DATS, despite its inhibition of the production of NO by macrophages, can significantly enhance the production of hydrogen peroxide (H₂O₂) by macrophages. When macrophages were pretreated with DATS for 24 h, the cytotoxicity % of macrophages to three tumor cell lines was significantly higher than that in corresponding control group (P<0.05,P<0.01). In the presence of both DATS and LPS, the cytotoxicity of macrophages was further enhanced so that the cytotoxicity % of macrophages to tumor cells was significantly higher than either that in the presence of DATS alone or that in the presence of LPS alone (P<0. 05,P<0. 01). These results indicate that DATS can augment the activation of T cells and enhance the anti-tumor function of macrophage, suggesting that DATS may be potentially useful in tumor therapy.

Keywords

diallyl trisulfide / T lymphocyte / macrophage / nitric oxide / hydrogen peroxide / tumor-derived immunosuppressive factor

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