Effect of transforming growth factor-β₂ on phagocytosis in cultured bovine trabecular meshwork cells

Abstract

The effect of transforming growth factor-β₂ (TGF-β₂) on phagocytosis in bovine trabecular meshwork cellsin vitro was investigated. After the cultured bovine trabecular meshwork cells were treated with 0 ng/ml, 0.32 ng/ml, 1 ng/ml, 3. 2 ng/ml TGF-β₂ for 24 h, latex beads were added into the incubation medium, and the numbers of the latex beads in 20 adjacent cells were counted under a microscope 24 h later, after treatment with Wright’s stain. Our results showed that the average numbers of the latex beads in the trabecular meshwork cells treated with TGF-β₂ of different concentrations were 53. 1±1. 7 beads/cell, 56. 4±2. 9 beads/cell and 77. 9±6. 5 beads/cell respectinvely, in comparison with 45. 5 ±3. 3 beads/cell of the control group. TGF-β₂ significantly increased the number of the latex beads phagocytosed by cultured bovine trabecular meshwork cells in a dose-dependent manner. TGF-β₂ could promote the phagocytosis of bovine trabecular meshwork cellsin vitro. It may be involved in the cellularity decrease of the trabecular meshwork in the patients of primary open angle glaucoma through promoting the phagocytosis of trabecular meshwork cells.

Keywords

transforming growth factor / trabecular meshwork / cultured cells / phagocytosis

References

[1] AlvaradoJ, MurphyC, JusterR, et al. . Trabecular meshwork cellularity in primary open angle glaucoma and nonglaucomatous normals. Ophthalmology, 1984, 91564-564

[2] GriersonI, HoggP. The proliferative and migratory activitues of trabecular meshwork cells. Prog Retinal Eye Res, 1995, 1533-33

[3] TripathiR C, LiJ, ChanW F, et al. . Aqueous humor in glaucomatous eyes contains an increased level of TGF-β₂. Exp Eye Res, 1994, 59723-723

[4] WangQ, WeiH, FanZ, et al. . Effect of norfloxacin and clonidine on human trabecular meshwork cellsin vitro. Graefe’s Arch Clin Exp Ophthalmol, 1994, 232566-566

[5] SherwoodM E, RichardsonT M. Phagocytosis by trabecular meshwork cells: sequence of events in cats and monkeys. Exp Eye Res, 1988, 46881-881

[6] ZhouL, FukuchiT, KawaJ, et al. . Loss of cell-matrix cohesiveness after phagocytosis by trabecular meshwork cells. Invest Ophthalmol Vis Sci, 1995, 36787-787

[7] ZhouL, LiY, YueB Y. Alteration of phagocytosis challenge in cells from an ocular tissue-the trabecular meshwork. In Vitro Cell Dev Biol Anim, 1999, 35144-144

[8] 2000,18:235

[9] LiJ, TripathiR C, TripathiB J. Modulation of pre-mR-NA splicing and protein production of fibronectin by TGF-beta2 in porcine trabecular cells. Invest Ophthalmol Vis Sci, 2000, 413437-3437

[10] 1999,35:186