Clinical significance and assessment of cytokines in various stages of ulcerative colitis

Abstract

In order to study the clinical significance and change of interleukin (IL)-1β and IL-10 concentration in intestinal mucosal tissues in various stage of ulcerative colitis (UC), IL-1β and IL10 levels were measured by enzyme linked immunosorbent assays (ELISA). Our results showed that IL-β level caused by spontaneous secretion in the intestinal mucous tissues in active stage of ulcerative colitis was significantly higher than that in normal controls and in remission stage of ulcerative colitis (P <0. 01,P<0. 001). IL-10 level in various stage of UC was relatively lower in controls, but there was no significantly difference between the two groups. Our study suggested that higher IL-1β level in active might play an important role in pathogenesis of UC, and IL-10, as an anti-inflammatory cytokine, was low in active UC, suggesting that it may be a important factor contributing to the development of higher IL-1β level.

Keywords

ulcerative colitis / cytokine

References

[1] HerfarthH H, BocherU, JanardhanamR, et al. . Subtherapeutic corticostevoids potentiate the ability of interleukin 10 to prevent chronic inflammation in rates. Gastroenterology, 1998, 115856-856

[2] StrongS A, PizarroT T, KleinJ S, et al. . Proinflammatory cytokines differentially modulate their own expression in human intestinal mucosal mesenchymal cells. Gastroenterology, 1998, 1141244-1244

[3] NikolausS, BauditzJ, GiouchettiiP, et al. . Increased secretion of pro-inflammatory cytkines by circulating Polymorphnuclear neutrophils and regulation by interleukin 10 during intestinal inflammation. Gut, 1998, 42470-470

[4] 1994,2(1)57

[5] MahidaY R, WuK, JewellD P. Enhanced production of interlukin-1β in mononuclear cells isolated from mucosa with active ulcerative colitis and crohn’s disease. Gut, 1989, 30835-835

[6] FiorentinoD F, ZlotnikA, VieiraP, et al. . IL-10 acts on the antigen presenting cell to inhibit cytokine production by Th1 cells. J Immunol, 1991, 146(10): 3444-3444

[7] NielsenO H, KoppenT, RudigerN, et al. . Involvement of interleukin 4 and interleukin 10 in inflammatory bowel disease. Dig Dis Sci, 1996, 41(9): 1786-1786

[8] SchreiberS, HeinigT, ThieveH G, et al. . Immunoregulatory role of interleukin 10 in patients with inflammatory bowel disease. Gastroenterology, 1995, 108(5): 1434-1434

[9] SteidlerL, HansW. Treatment of murine colitis by Iactococcus Iactis secreting interleukin-10. Science, 2000, 289352-352