Construction andin vitro expression ofStreptococcus mutans surface protein encoding DNA vaccine

Abstract

DNA vaccine plasmids were constructed that encoded two highly-conservative regions of a surface protein, PAc, from the human major cariogenic bacterium,Streptococcus mutans. Antigen expression was evaluatedin vitro by immunohistochemical analysis of human endothelial cells following cationic liposome-mediated transient transfection with recombinant plasmid. The results of this study provided a basis for further testing of these recombinant plasmids in primates and for efficacy testing of dental caries DNA vaccines in human volunteers in future.

Keywords

Streptococcus mutans / surface protein / DNA vaccine

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