The role of neutrophil collagenase in endotoxic acute lung injury

Abstract

The aim of this study was to determine the role of neutrophil collagenase in the pathogenesis of acute lung injury induced by endotoxin. 28 Sprague-Dawley were randomized into control group and LPS-enduced groups. Samples of left lung were obtained in 2 h (group L₁), 6 h (group L₂), 12 h (group L₃) after intravenous LPS. Immunohistochemsitry was employed for detection of expression of neutrophil collagenase. Pathological scores, lung wet/dry weight ratio and the number of neutrophils were measured. The results showed that the concentration of neutrophil collagenase in LPS-enduced groups (group L₁, L₂, L₃) were significantly higher than that of control group (P<0.01). Pathological scores, lung wet/dry weight ratio and the number of neutrophils in LPS-enduced groups (group L₁, L₂, L₃) were also significantly higher than that of control group (P<0.01). Moreover, among group L₁, L₂ and L₃, there were significant correlations in concentration of neutrophil collagenase and pathological scores, lung wet/dry weight ratio, the number of neutrophils (P<0.05). The present study showed that neutrophil collagenase play an important role in the pathogenesis and progress of endotoxic acute lung injury.

Keywords

acute lung injury / endotoxin / neutrophil / collagenase

References

[1] D'OrthpM P, JarreauP H, DelacourtC, et al. . matrix metalloproteinase and elastase activities in LPS-induced acute lung injury in guinea pigs. Am J Physiol, 1994, 266(9): L209-L216

[2] TomashefskiJ F. Pulmonary pathology of the adult respiratory distress syndrome. Clin Chest Med, 1990, 11(2): 593-593

[3] TamuraD Y, MooreE E, PartrickDA, et al. . Acute hypoxemia in humans enhances the neutrophil inflammatory response. Shock, 2002, 17(11): 269-269

[4] WuE X, WangF F, NormanM. Metalloproteinases. Res Life Sci, 1999, 3(1): 175-175

[5] TorllK, LidaK, MiyazakiY, et al. . higher concentrations of matrix metalloproteinases in bronchoalveolar lavage fluid of patients with adult respiratory distress syndrome. Am J Respir Care Med, 1997, 155(2): 43-46

[6] RicouB, NlcodL, LacrazS, et al. . Matrix metalloproteinases and TIMP in acute respiratory distress syndrome. Am J Respir Care Med, 1996, 154(11): 346-346

[7] SassiM L. Carboxyterminal degradation products of type I collagen, 2001, Finland, Oulu University Press

[8] RuthS, YrjoT, YangL D, et al. . Human neutrophil collagenase (MMP-8), identified in brochiectasis BAL fluid, correlates with severity of disease. Chest, 1995, 107(6): 1641-1641