Effect of exercise on the expression of adiponectin mRNA and GLUT4 mRNA in type 2 diabetic rats

Abstract

To investigate the impact of exercise on the expression of adiponectin and GLUT4 mRNA in type 2 diabetic rats, type 2 diabetic rat model was made. The diabetic rats were treated with swimming training for 8 weeks. The expression of adiponectin mRNA in perirenal fat and GLUT4 mRNA in skeletal muscles were assessed by reverse transcription polymerase chain reaction (RT-PCR) and the levels of blood glucose, serum insulin, and blood lipid were measured. Our results showed that the expression of adiponectin mRNA and GLUT4 mRNA in diabetic model group was decreased by 45% (P<0.01), 43% (P<0.01) respectively. The gene expression of adiponectin and GLUT4 was increased significantly in swimming group (P<0.05 andP<0.01, respectively). Compared with the model group, fasting insulin, TG, TC and FFA were decreased significantly in the training group (P<0.05 orP<0.01) as compared with model group. It is concluded that exercise can promote the expression of adiponectin mRNA and GLUT4 mRNA in type 2 diabetic rats, which may be one of the mechanisms responsible for the amelioration of insulin resistance in the rats.

Keywords

type 2 diabetes / insulin resistance / adiponectin / GLUT4 / exercise

References

[1] XieM Z, LiuH F, ZhangL Y, et al. . An experimental rat model of obesity and diabetes. Acta Pharmaceutica Sinica (Chinese), 1985, 20(11): 801-801

[2] PlougT, StallknechtB M, PedersenO, et al. . Effect of endurance training on glucose transport capacity and glucose transporter expression in rat skeletal muscle. Am J Physiol, 1990, 259E778-E778

[3] JonsdottirI H, SchjerlingP, OstrowskiK, et al. . Muscle contractions induce interleukin-6 mRNA production in rat skeletal muscles. J Physiol, 2000, 528157-157

[4] LiG W, PanX Y, lilliojaS, et al. . A new index to check insulin sensitivity in public. Chin Med J, 1993, 32(10): 656-656

[5] YamauchiT, KamonJ, MinokoshiY, et al. . Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase. Nat Med, 2002, 8(11): 1288-1288

[6] WuX D, MotoshimaH, MahadevK, et al. . Involvement of of Adiponectin in Primary Rat Adipocytes. Diabetes, 2003, 521355-1355

[7] LihnA S, JessenN, PedersenS B, et al. . AICAR stimulates adiponectin and inhibits cytokines in adipose tissue. Biochem Biophys Res Commun, 2004, 316(3): 853-853

[8] NicolasM, NobuharuF, MichaelF, et al. . Amp-activated protein kinase (ampk) is activated in muscle of subjects with type 2 diabetes during exercise. Diabetes, 2001, 50921-921

[9] MaretteA, AtgieC, LiuZ. Differential regulation of GLUT1 and GLUT-4 glucose transporter capacity in skeletal muscle of a new model of type II diabetes, the obese SHR/N-cp rat. Diabetes, 1993, 421195-1195