Detection of DNA aneuploidy in exfoliated airway epithelia cells of sputum specimens by the automated image cytometry and its clinical value in the identification of lung cancer

Abstract

To evaluate the value of detection of DNA aneuploidy in exfoliated airway epithelia cells of sputum specimens by the automated image cytometry for the identification of lung cancer, 100 patients were divided into patient group (50 patients with lung cancer) and control group (30 patients with tuberculosis and 20 healthy people). Sputum was obtained for the quantitative analysis of DNA content of exfoliated airway epithelial cells with the automated image cytometry, together with the examinations of brush cytology and conventional sputum cytology. Our results showed that DNA aneuploidy (DI>2.5 or 5c) was found in 20 out of 50 sputum samples of lung cancer, 1 out of 30 sputum samples from tuberculosis patients, and none of 20 sputum samples from healthy people. The positive rates of conventional sputum cytology and brush cytology were 16% and 32%, which was lower than that of DNA aneuploidy detection by the automated image cytometry (P<0. 01,P>0.05). Our study showed that automated image cytometry, which uses DNA aneuploidy as a marker for tumor, can detect the malignant cells in sputum samples of lung cancer and it is a sensitive and specific method serving as a complement for the diagnosis of lung cancer.

Keywords

automated image cytometry / lung cancer / DNA aneuploidy / sputum

References

[1] PeterW, PayneR T, ThomasJ S, et al. . Sputum by quantitative microscopy: a reexamination of a portion of the national cancer institute cooperative early lung cancer study. Mayo Clinc Proc, 1997, 72(8): 697-697

[2] GagnetenC B, GellerG E, SaenzM C. Diagnosis of bronchogenic carcinoma through the cytologic examination of sputum with special reference to tumour typing. Acta Cytol, 1970, 20530-530

[3] JohnstonW W. Cytologic diagnosis of lung cancer: Principle and problems. Pathol Res Pract, 1986, 1811-1

[4] MarekW, KrampeS, et al. . Automatisierte quantitative image-Zytometrie bronchialer spülflüssigkeiten bei Verdacht auf broncho-pulmonale Tumoren: Vergleich mit Zytologie, Histologie und klinischer Diagnose. Pneumologie, 1999, 53583-583

[5] MarekeW, Kotschy-LangN, MutiA, et al. . Can Semiautomated image cytometry on induced sputum become a screening tool for lung cancer?. Euro Respir J, 2001, 18942-942

[6] SchulteE, WittekindD H. Standarization of the Feulgen reaction: influence of chromatin condensation on the kinetics of acid hydstoltsis. Anal Cell Pathol, 1990, 2149-149

[7] AuerC, CasperssonT, WallgrenA. DNA content and survival in mammary carcinoma. Anat Quant Cytol, 1980, 3161-161

[8] BiesterfeldS, GerresK, et al. . Polyploidy in non-neoplastic tissue. Clin Pathol, 1994, 4738-38