Anti-endotoxic effects of syringic acid ofRadix Isatidis

Abstract

The anti-endotoxic effect of syringic acid (SA) isolated fromRadix Isatidis (Banlangen, BLG) was studied. SA was extracted and isolated from BLG and diluted into 1% solution. The content of SA-pretreated endotoxin (ET) was quantitatively determined using Limulus test. The ability of fever induction of ET pretreated with SA was measured using endotoxin-induced fever test in rabbits. The LPS-induced death in mice pretreated with and without SA was compared. Results showed that after pretreatment with SA, 83.16% of ET was destroyed, the ET-induced fever in rabbits relieved markedly and the LPS-induced death rate in mice dropped from 68 % to 20 %. It was concluded that SA isolated from BLG had anti-endotoxic effects.

Keywords

syringic acid / anti-endotoxin / Radix Isatidis / endotoxin / lipopolysacchrides / Tachypleus Amebocyte Lysale / mortality

References

[1] WuXY, LiuY H, ShengW Y, et al. . Chemical constituents of Isatis Indigotical. Planta Medica, 1997, 6355-55

[2] VogelS N, MooreR N, SipeJ D, et al. . BCG-induced enhancement of endotoxin sensitivity in C3H/HeJ mice. J Immunol, 1980, 124(10): 2004-2004