Experimental study on inhibition of neuronal toxical effect of Levodopa by Ginkgo Biloba Extract on Parkinson disease in rats

Abstract

In order to observe neuronal toxical effect of Levodopa and investigate if using Levodopa together with Ginkgo Bilobar Extract (EGb) would be an workable method to treat Parkinson disease, rat models of Parkinson disease (PD) were made by injecting 6-OHDA stereotaxically to right side of the mesencephic ventral tegmental area (VTA) and substantia nigra pars compacta (SNc). Rotational behavioral observation, TUNEL, immunocytochemistry, Nissl’s body staining were performed to measure the difference between group treated by Levodopa (50 mg/kg every day for 3 days, 5 days, 7 days, L-dopa group) and group treated by Levodopa combined with EGb (100 mg/kg every day, E-D group). The results showed that in the L-dopa group, the numbers of apoptosis of substantial nigra, rings of rotational behavior were more than those in the E-D group (P<0.05). The numbers of Nissl’s cells in L-dopa group were fewer than in E-D group (P<0.05). The results suggested that Levodopa had neur toxic effect and EGb may decrease the toxicity of levodopa. The combined use of EGb with Levodopa may be a workable method to treat PD and may be better than using Levodopa alone.

Keywords

ginkgo biloba extract / apoptosis / L-dihydroxy-phenylalanine

References

[1] TrugmanJ M, HubbardC A, BennettJ J. Dose-related of continuous levodopa infusion in rat with unilateral lesions of the substantia nigra. Brain Res, 1996, 725177-177

[2] ThomasJ, WangJ, TakubaH, et al. . A 6-OHDA induced selective Parkinsonian rat model: further biochemical and behavioural characterization. Exp Neurol, 1994, 126159-159

[3] MelamedE, OffenD, ShirvanA, et al. . Levodopa toxicity and apoptosis. Ann Neurol, 1998, 44(3 Suppl 1): 149-149

[4] JennerP G, BrinM F. Levodopa neurotoxicity: experimental studies versus clinical relevance. Neurology, 1998, 50(6 Suppl 6): 44-44

[5] SastreJ, PallardoF V, de la GarciaAsuncion, et al. . Mitochondria, oxidative stress and aging. Free Radic Res, 2000, 32(3): 189-189

[6] MayoJ C, SainzR M, AntolinI, et al. . Ultrastructural confirmation of neuronal protection by melatonin against the neurotoxin 6-hydroxydopamine cell damage. Brain Res, 1999, 818(2): 221-221

[7] NiY, ZhaoB, HouJ, et al. . Preventive effect of Ginkgo biloba extract on apoptosis in rat cerebellar neuronal cells induced by hydroxyl radicals. Neurosci Lett, 1996, 214115-115