Study of rat osteoblasts transfected by transforming growth factor β₁ gene

Abstract

In order to investigate the effect of TGFβ₁ gene transfer on the biological characteristics, the effects of gene transfer and supernatant of transfected osteoblasts on the proliferation and ALP activity of osteoblasts were detected by³H-TdR and MTT. Our results showed that TGFβ₁ gene transfer had no effect on the biological characteristics and the activated supernatant of transfected osteoblasts stimulated proliferation and inhibited ALP activity of osteoblasts. TGFβ₁ gene transfer could promote the expression of TGFβ₁ and the biological characteristics of transfected osteoblasts were stable, which might be helpful for gene therapy of bone defectsin vivo.

Keywords

transforming growth factor β₁ / osteoblasts / gene transfer

References

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