Standardization of lymphocyte transformation to antigen

Abstract

In vitro lymphocyte transformation to non-specific stimulant or specific stimulant is frequently used in the assessment of cell-mediated immunity so that a better understanding of the immune response must be gained before use. Lymphocyte transformation to bovine serum albumin (BSA) or human serum albumin (HSA) immunogen was carefully studied. The standard conditions were: (1) 100 μg of BSA or HSA immunogen; (2) 120 hours of incubation time after addition of antigen; (3) 1/30 dilution of whole blood and 0.5X10⁶ lymphocytes per culture; (4) 1 μCi of³H-thymidine per culture.

Keywords

lymphocyte transformation / cell-mediated immunity (CMI) / ³H-thymidine / standardization

References

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