Cell attachment of periodontal ligament cells on commercially pure titanium at the early stage

Abstract

In order to study the character of periodontal ligament cells (PDLCs) attaching on commercially pure titanium (cpTi) by morphology and metrology on the early stage (24 h), 1×10⁵/ml PDLCs in 2 ml culture medium were seeded on cpTi discs fixed in 24-well culture plates. Morphology of cell attachment was observed by contrast phase microscope, scanning electron microscope (SEM) and fluoroscence microscopy. Cell adhesion was analyzed by MTT at 0.5, 1, 2, 4 h respectively. PDLCs could attach and spread on cpTi discs. SEM showed that PDLCs had pseudopod-like protuberance. PDLCs showed different attaching phases and reached saturation in cell number at 2 h. It was concluded that PDLCs had good biocompatibility with cpTi, and showed a regular and dynamic pattern in the process of attaching to cpTi.

Keywords

periodontal ligament cell / commercially pure titanium / cell attachment

References

[1] PuleoD A, NanciA. Understanding and controlling the bone-implant interface. Biomaterials, 1999, 202311-2311

[2] GiovannaO, BartolimeoA, AntonioS, et al. . Surface analysis of machined versus sandblasted and acid-etched titanium implants. Int J Oral Maxillofacial Implants, 2000, 15779-779

[3] ChoiB H. Periodontal ligament formation around titanium implant using cultured periodontal ligament cells. Int J Oral Maxillofac Implont, 2000, 15193-193

[4] LinD G, KennyD J, LekicP. Storage conditions of avulsed teeth effect the phenotype of cultured human periodontal ligament cells. J Periodont Res, 2000, 3542-42

[5] YliheikkiläP K, FeltonD A, WillianW S, et al. . Correlative microscopic investigation of the interface between titanium alloy and the osteoblast-osteoblast matrix using mineralizing cultures of primary fetal bovine mandibular osteoblasts. Int J Maxillofacial Implants, 1995, 10655-655