Expression of CC chemokine ligand 20 and CC chemokine receptor 6 mRNA in patients with psoriasis vulgaris

Abstract

In order to explore the possible role of CC chemokine ligand 20 (CCL20) and its receptor CC chemokine receptor 6 (CCR6) in the pathogenesis of psoriasis, the expression levels of mRNA of them in psoriatic lesions were investigated. The skin biopsies were collected from skin lesions in 35 cases of psoriasis vulgaris and 18 normal controls. RT-PCR was used to semi-quantitatively analyze the mRNA expression of CCL20 and CCR6 in the psoriatic lesions and the normal skin tissues. The results showed that the mRNA of CCL20 and CCR6 was present in every specimen. The expression levels of CCL20 mRNA in skin lesions were 1.1397±0.0521, which were greatly higher than those in normal controls (0.8681±0.0308) (P<0.001). The expression levels of CCR6 mRNA in skin lesions were 1.1103±0.0538, significantly higher than in the controls (0.9131±0.0433,P<0.001). These findings indicate that up-regulated expression of CCL20 and CCR6 mRNA might be related to the pathogenesis of psoriasis.

Keywords

psoriasis / CC chemokine ligand 20 / CC chemokine receptor 6

References

[1] TeranishiY, MizutaniH, MurataM, et al. . Increased spontaneous production of IL-8 in peripheral blood monocytes from the psoriatic patient: relation to focal infection and response to treatments. J Dermatol Sci, 1995, 10(1): 8-8

[2] RossiD L, VicariA P, Franz-BaconK, et al. . Identification through bioinformatics of two new macrophage proinflammatory human chemokine: MIP-3alpha and MIP-3beta. J Immunol, 1997, 1581033-1033

[3] TanakaY, ImaiT, BabaM, et al. . Selective expression of liver and activation-regulated chemokine (LARC) in intestinal epithelium in mice and humans. Eur J Immunol, 1999, 29(2): 633-633

[4] Dieu-NosjeanM C, MassacrierC, HomeyB, et al. . Macrophage inflammatory protein 3alpha is expressed at inflamed epithelial surfaces and is the most potent chemokine known in attracting Langerhans cell precursors. J Exp Med, 2000, 192(5): 705-705

[5] UyemuraK, YamamuraM, FivensonD F, et al. . The cytokine network in lesional and lesion-free psoriatic skin is characterized by a T-helper type 1 cell-mediated response. J Invest Dermatol, 1993, 101(2): 701-701

[6] SchonY, DetmarM, ParkerC M, et al. . Animal models of psoriasis: what can we learn from them?. J Invest Dermatol, 1999, 1129(4): 405-405

[7] DeleuranM, BuhlL, EllingsenT, et al. . Localization of monocyte chemotactic and activating factor (MCAF/MCP-1) in psoriasis. J Dermatol Sci, 1996, 13(3): 228-228

[8] HomeyB, Dieu-NosjeanM C, WiesenbornA, et al. . Upregulation of macrophage inflammatory protein-3 alpha/CCL20 and CC chemokine receptor 6 in psoriasis. J Immunol, 2000, 164(12): 6621-6621

[9] ChabaudM, PageG, MiossecP, et al. . Enhancing effect of IL-1, IL-17, and TNF-alpha on macrophage inflammatory protein-3alpha production in rheumatoid arthritis: regulation by soluble receptors and Th2 cytokines. J Immunol, 2001, 167(10): 6015-6015

[10] AlbanesiC, ScarponiC, CavaniA, et al. . Interleukin-17 is produced by both Th1 and Th2 lymphocytes, and modulates interferon-gamma- and interleukin-4-induced activation of human keratinocytes. J Invest Dermatol, 2000, 115(1): 81-81