Role of nuclear factor kappaB in intestine injury induced by hepatic ischemia reperfusion

Abstract

The role of nuclear factor kappaB in intestine injury induced by hepatic ischemia reperfusion was investigated. Eighteen male Wistar rats were divided into 3 groups randomly: sham operation group (group A), hepatic ischemia reperfusion group (group B) and hepatic ischemia reperfusion plus pyrrolidine dithiocarbamate (PDTC) group (group C). The rats in group A were only subjected to laparotomy, those in group B underwent partial hepatic ischemia reperfusion (ischemia for 1 h and reperfusion for 2 h) and those in group C underwent the same procedure as that of group B but received PDTC 200 mg/kg i. v. before and after ischemia. After reperfusion, tissues of jejunum and venous blood were obtained for measurement of TNF-α, MDA and MPO. The levels of TNF-α in jejunum and venous blood, the levels of MPO in jejunum in group B were significantly higher than those in group A and group C (P<0.05). There was no significant different in the levels of MDA between group B and group C. The severity of histological intestinal injury in group B and group C was similar. Hepatic ischemia reperfusion caused intestine injury, NF-kappaB may play an important role in this course and the targeting of upstream components of the inflammatory response, such as NF-kappaB, may have important therapeutic applications.

Keywords

liver / ischemia reperfusion / nuclear factor kappaB / rats

References

[1] LiuD L, JeppssonB, HakanssonC H, et al. . Multiplesystem organ damage resulting from prolonged hepatic flow interruption. Arch Surg, 1996, 131442-442

[2] GhoshS, MayM J, KoppE B, et al. . Rel proteins: evolutionarily conserved mediators of immune responses. Annu Rev Immunol, 1998, 16225-225

[3] KobayashiH, NonamiT, KurokawaT, et al. . Mechanism and prevention of ischemia reperfusion induced liver injury in rats. J Surg Res, 1991, 51240-240

[4] LentschA B, YoshidomeH, CheadleW G, et al. . Chemokine involvement in hepatic ischemia reperfusion injury in mice: roles for macrophage inflammatory protein 2 and KC. Hepatology, 1998, 271172-1172

[5] PeraltaC, FernandezL, PanesJ, et al. . Preconditioning protects against systemic disorders associated with hepatic ischemia reperfusion through blockade of tumor necrosis factor-α induced P-selectin up-regulation in the rat. Hepatology, 2001, 33100-100

[6] CollinsT, ReadM A, NeishA S, et al. . Transcriptional regulation of endothelial cell adhesion molecules: NF-κB and cytokine-inducible enhancers. FASEBJ, 1995, 9899-899

[7] ZwackaR M, ZhangY, ZhouW, et al. . Ischemia reperfusion injury in the liver of BALB/c mice activates AP-1 and nuclear factorκB independently of IkB degradation. Hepatology, 1998, 281022-1022

[8] Van AntwarpD J, MartinS J, VermaI M, et al. . Inhibition of TNF induced apoptosis by NF-κB. Trends Cell Biol, 1998, 8107-107