Genetic polymorphisms analysis of glutathione S-transferase M1 and T1 in children with acute lymphoblatic leukemia

Abstract

The relationship between glutathione S-transferases (GSTs) M1, T1 genotype and childhood acute lymphoblastic leukemia (ALL) was investigated. GSTM1 and GSTT1 genotypes in genomic DNA from 67 children with ALL and 146 healthy controls were analyzed by using the multiplex polymerase chain reaction (PCR). The frequencies of GSTM1, M1-T1 null genotypes in ALL children were significantly higher than in the healthy controls (76. 12% versus 52.74%. OR=2. 856.P<0.001: 50.74% versus 24.66%, OR=3.148,P<0.01, respectively). However, there was no significant relationship between GSTT1 null genotype and ALL of children (61.19% versus 49.32%. OR=1.621.P>0.05). It was suggested that GSTM1 null genotype might be a risk genotype of childhood ALL. While there as no correlation between GSTT1 null genotype and childhood ALL.

Keywords

gene / polymorphisms / glutathione S transferase

References

[1] WhalenR, BoyerT D. Human glutathione S-transferase. Semin Liver Dis, 1998, 18(4): 345-345

[2] RebbeckT R. Molecular epidemiology of the human glutathione S-transferase genotypes GSTM1 and GSTT1 in cancer susceptibility. Cancer Epidemiol Biomarkers Prev, 1997, 6(9): 733-733

[3] LehrnbecherT, FosterC B, ZhuS, et al. . Variant genotypes of the low-affinity Fey receptors in two control populations and a review of low affinity Fey receptor polymorphism in control and disease populations. Blood, 1999, 94(12): 4220-4220

[4] ArandM, MuhlbauerR, HengstlerJ, et al. . A multiplex polymerase chain reaction protocol for simultaneous a-analysis of the glutathione S-transferase GSTM1 and GSTT1 polymorphisms. Anal Biochem, 1996, 236(1): 184-184

[5] HayesJ D, PulfordD J. The glutathione S-transferase supergene family: regulation of GST and contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol, 1995, 30(6): 445-445

[6] SaadatI, SaadatM. The glutathione S-transerase mupolymorphism and susceptibility to acute lymphocytic leukemia. Cancer Lett, 2000, 158(1): 43-43

[7] RojasM, CascorbicI, AlexandrovK, et al. . Modulation of benzo[α] pyrene diolepoxide-DNA adduct levels in human white blood cells by CYP1A1, GSTM1 and GSTT1 polymorphism. Carcinogenesis, 2000, 21(1): 35-35

[8] RojasM, AlexandrovK, CascorbiI, et al. . High benzo [α] pyrene diol-epoxide DNA adduct levels in lung and blood cells from individuals with combined CYP1A1 MspI/MspI, GSTM1 0/0 genotypes. Pharmacogentics, 1998, 8(2): 109-109