Preparation and characterization of polyclonal antibodies against VLDL receptor

Abstract

The polyclonal antibodies against VLDL receptor were prepared and identified. Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The collected blood serum of the immunized rabbits was analyzed and identified by using ELISA and Western Blot. The results showed that the rabbit against mouse and human VLDL receptor antibodies were obtained with high titer and could recognize the natural VLDL receptors through Western blot. The prepared polyclonal antibodies against VLDL receptor provide a new tool to study the protein of VLDL receptor.

Keywords

VLDL receptor / polyclonal antibodies

References

[1] HiltumonT P, LuomaJ S, NikkariT, et al. . Expression of LDL receptor, VLDL Receptor, LDL receptor-related protein and scavager receptor in rabbit atherosclerosis lesions: marked induction of scavenger receptor and VLDL receptor expression during lesion development. Circulation, 1998, 97(11): 1079-1079

[2] RotenbergerP M, OkaK, PetersenH H, et al. . Ligand binding properties of the very low density lipoprotein receptor. J Bio Chem, 1999, 2748973-8973

[3] HiroakiI, TokuoT Y. Expression and characterization of a very low density lipoiprotein receptor variant lacking the o-linked sugar region generated by alternative splicing. J Bio Chem, 1998, 124747-747

[4] HarlowE, LaneD. Antibodies: A Laboratory Manual, 1988, NY, Cold Spring Harbor Press: 138-138

[5] BaD N. Mordern immundogical technology and application, 1998, Beijing, Beijing Medical Uniersity and Chinese Union Medical University press: 328-328

[6] TiebelO, OkaK, SulivanM, et al. . Mouse very-low-density-lipoprotein receptor (VLDLR): gene structure, tissue-specific expression and dietary and developmental regulation. Atherosclerosis, 1999, 145239-239

[7] NakamuraY, YamamotoM, KumamaruE. Very low density lipoprotein receptor in fetal intestine and gastric adenocarcinoma cells. Arch Pathol Lab Med, 2000, 124119-119