Transfection efficiency comparison of oligonucleotide and plasmid to the HL-60 cell line with liposomes

Abstract

The transfection efficiency of oligonucleotide and plasmid to the HL-60 cell line with lipofectaminedPLUS was compared through observing the transfection rate and the expression duration of exogenous gene in the target cells. The results showed that the transfection rate of oligonucleotide to the HL-60 was about 90%–95% and it had no obvious attenuation within 84h. However, the plasmid transfection rate was only 5%–25% and it was decreased significantly within 60h. It was suggested that the transfection of oligonucleotide with liposomes was better than that of plasmid.

Keywords

HL-60 cell line / liposomes / plasmid / oligonucleotide chain

References

[1] TangZ S. Molecular Surgery and Gene Therapy (Chinese), 1999, Shanghai, Publishing House of Shanghai Medical University: 8-15

[2] ZhouJ F, LiuW L, TangY, et al.. Establishment of the cell line U937-ASPI3K with stable expression of antisense ATM/PI3K. J Exp Hematol (Chinese), 2001, 9(1): 23-23

[3] TsengW, PurvisN B, HaseltonF R, et al.. Cationic liposomal delivery of plasmid to endothelial cells measured by quantitative flowcytometry. Biotechnol Bioeng, 1996, 50(5): 548-548

[4] WengW Y, XuH N, ZhuangQ Q. The non-virus mediated gene therapy system. Guowai Yixue Hechengyao Shenghuayao Zhiji Fence (Chinese), 2000, 21(2): 72-72