Analysis of AmpC β-lactamase gene inPseudomonas aeruginosa

Abstract

The gene and the amino acid sequence of the structural and regulatory region of thePseudomonas aeruginosa with different resistance patterns were analyzed. Six strains with different resistance patterns were selected and the AmpC β-lactamase was identified. The objective gene fragment was amplified by colonies PCR. The sequences of the PCR-products were analyzed. The DNA sequence of the structural gene ampC and the regulatory genes ampR, ampD and ampE was detected. The 6 strains and the wild-typePseudomonas aeruginosa are highly homogeneous in structural and regulatory region. Some new mutant points were found.

Keywords

Pseudomonas aeruginosa / β-lactamase / AmpC β-lactamase

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