Relationship between single nucleotide polymorphism in TNF-α gene promoter region and inibitory effects of triptolide on TNF-α production in peripheral blood mononuclear cells of healthy humans

Abstract

The relationship between tumour necrosis factor-α (TNF-α) gene polymorphism and inhibitory effects of triptolide on TNF-_α production from perlpheral blood mononuclear cells (PBMC) of healthy humans was investigated. Genomic DNA from 41 healthy people was typed for TNF-_α—308 polymorphism by allele-specific polymorphism chain reaction (AS-PCR). The TNF-_α concentration in the supernatant was measured by ELISA. The results showed that the production of TNF-_α from TNF-_α—308 non-G/G genotype PBMC was higher than that from TNF-_α—308 G/G genotype PBMC after stimulated by LPS. Triptolide could lower the production of TNF-_α from G/G genotype PBMC, but had no effect on the level of TNF-_α from non-G/G genotype PBMC. It was concluded that TNF-_α gene polymorphism was related to the TNF-_α production from triptolide-inhibited PBMC culture in healthy humans.

Keywords

tumor necrosis factor-_α / gene polymorphism / triptolide / peripheral blood mononuclear cells

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