Effect of electroacupuncture on reperfusion ventricular arrhythmia in rat

Abstract

Protective effect and mechanism of electroacupuncture (EA) on acute reperfusion ventricular arrhthmia was investigated. Ventricular arrhythmia was induced by occlusion of the proximal left anterior descend (LAD) branch of coronary artery for 5 min and followed with 15 min reperfusion. EA on acupoint “Neiguan”, “Jianshi” was performed at 30 min before ligation and continued another 5 min during ischemia. Isoprenaline (20, 30 and 50 μg/kg) or atropine (1 mg/kg) was intravenously injected at 5min before ischemia. The results showed that EA significantly decreased the incidence of ischemia/reperfusion (I/R) induced ventricular tachycardia (VT), ventricular fibrillation (VF) and mortality as compared to I/R group. Atropine partially suppressed the EAs effect of antiarrhythmia; Isoprenaline increased the incidence and severity of reperfusion arrhythmia, which was inhibited by EA, but this inhibition of EA was blocked with increasing dose of isoprenaline. The results indicated that EA treatment could prevent the occurrence of reperfusion ventricular arrhythmia in rats with myocardial ischemia, and its mechanism might be related to the regulation of EA on the β-adrenoceptors and M-cholinergic receptor activation in myocardium.

Keywords

acute myocardial ischemia / ventricular arrhythmia / electroacupuncture / β-adrenoceptors / M-cholinergic receptor

References

[1] TianY F. Overview of the protection effect of acupuncture Nei Guan on the heart (Chinese). J Clinic Acupuncture, 1999, 15(10): 50-52

[2] LiuZ, ChenY, ShiB, et al.. Experimental studies of electroacupuncture on ventricular fibrillation threshold in rats with acute ischemic myocardium. J Tongji Med Univ, 1998, 18(2): 90-93

[3] CincaC J. Reperfusion arrhythmias during coronary thrombolysis: clinical reality or experiment curiosity?. Rev Esp Cardiol, 1992, 45(suppl2): 60-65

[4] ManningA S, HearseD J. Reperfusion-induced arrhythmia: mechanisms and prevention. J Mol Cell Cardiol, 1984, 16: 497-518

[5] TaoZ L, XiS Y, ZhangZ P, et al.. The segmental distribution of the afferent neurons of the afferent neurons of the “Jian Shi” point. Acupuncture Research (Chinese), 1986, 11(1): 40-43

[6] RadzievskiiS A, VorontsovaE Ia. The effect of electroacupuncture and acupuncture on ischemic and reperfusion cardiac arrhythmias. Vopr Kurortol Fizioter Lech Fiz Kult, 1989, 6: 7-9

[7] SchomigA, RichardtG, KutzT. Sympatho-adrenergic activation of the ischemic myocardium and its arrhythmogenic impact. Herz, 1995, 20(3): 169-186

[8] De FerrariG M, VanoliE, CurcurutoP, et al.. Prevention of life-threatening arrhythmias by pharmacologic stimulation if the muscarinic receptors with oxotremorine. Am Heart J, 1992, 124(4): 8883-8890

[9] RosenshtraukhL, DaniloP, AnyukhovskyE P, et al.. Mechanisms for vagal modulation of ventricular repolarization and of coronary occlusion-induced lethal arrhythmia in rat. Circ Res, 1994, 75(4): 722-732

[10] LiuR T. Observation of the influence of acupuncture on experimental acute myocardial ischemia of dogs. Traditional Chinese Medicine (Chinese), 1980, 21(5): 77-77