Correlation between cyclin A gene expression in adult patients with acute leukemia and drug resistance

Abstract

In order to investigate the relationship between the expression of cyclin A and drug resistance in adult patients with acute leukemia (AL), the mRNA expression of cyclin A, mdr1, Top II_α, bcl-2 was detected in 64 adult patients with AL and 20 normal controls by semi-reverse transcription polymerse chain reaction (semi-RT-PCR). It was found that the cyclin A and Top II_α mRNA expression levels in drug resistant group were significantly lower than in sensitive group (P<0.01). Under the same experimental condition no cyclin A mRNA expression was detectable in all normal controls. The mdr1 and bcl-2 mRNA expression levels in resistant group were significantly higher than in sensitive group (P<0.01). cyclin A and Top II_α gene expression levels were closely correlated (r₃=+0.794,P=0.000,n=64) in all AL patients, but cyclin A was not correlated with mdr1 and bcl-2 gene expression levels. In drug resistant group there was a negative correlation between the gene expression levels of cyclin A and mdr1 (r₃=−0.337,P=0.029). The 10 AL patients with positive lower expression of both cyclin A and Top II_α were all resistant to drugs. Logistic regression of Binary analysis showed the correlation between the lower expression of cyclin A and drug resistance. It was concluded that lower expression of cyclin A gene might be an unfavorable prognostic factor for patients with AL, and detection of both cyclin A and Top II_α gene expression would predict drug resistance in AL patients.

Keywords

acute leukemia / cyclin A / drug resistance / multiple

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