Synthesis of CCK-8 tetrapeptide fragment by enzymatic method

Abstract

The enzymatic synthesis of a tetrapeptide Phac-Met-Gly-Trp-Met-OEt, a fragment of the cholecystokinin C-terminal octapeptide CCK-8, was reported. This fragment was synthesized by coupling Phac-Met-OEt with Gly-OMe, Trp-OMe and Met-OEf successively. These three steps were catalyzed by α-chymotrpsin, Papain and α-chymotrpsin respectively. The results of FAB-MS showed that all the products had the correct molecular mass.

Keywords

CCK-8 / enzymatic method / α-chymotrpsin / papain

References

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