Effects of L-tetrahydropalmatine on NOS III gene expression in hypoxia and cultured porcine cerebral arterial endothelial cells during reoxygenation

Abstract

To investigate the expression of NOS III mRNA and protein in cultured porcine cerebral arterial endothelial cells (CAEC) during hypoxia and reoxygenation and the effects of L-Tetrahydropalmatine (L-THP) on the gene expression of NOS III in CAEC during hypoxia and reoxygenation. The cultured CAEC were divided into 5 groups; control, hypoxia, hypoxia+reoxygenation, hypoxia +L-THP and reoxygenation +L-THP groups. NOS III mRNA expression was detected by reverse transcription-polymerase chain reaction (RT-PCR). Immunocytochemistry was used to detect the level of NOS III protein. The expression of NOS III mRNA and protein were increased when CAEC were exposed to hypoxia for 1 h, and significantly decreased during reoxygenation 2, 6 and 12 h after 1-h of hypoxia. L-THP from 10⁻⁸ mol/L to 10⁻³ mol/L could inhibit the up-regulation of NOS III gene expression during hypoxia and down-regulation of NOS III gene expression during reoxygenation.

Keywords

cerebral arterial endothelial cell / NOS III / L-THP / hypoxia and reoxygenation

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